Abstract:A method for the determination of 17 sulfonamides(SAs)in aquatic products by ultra performance liquid chromatography(UPLC)with photodiode array(PDA)detector was developed.The SAs in the samples were first extracted with ethyl acetate，then the ethyl acetate extraction was purified and concentrated by HLB cartridges.The analyses were performed on UPLC with an ACQUITY UPLC BEH C18column(2.1 mm×100 mm,1.7 μm),and the mobile phase was methanol 0.1％ acetic acid aqueous solution.SAs were separated by gradient elution and detected by PDA within 8 min.This method showed a good linearity.For sulfacetamide,sulfadiazine and sulfisomidine,the linearity was in the range of 0.01~2 μg/ml,and for the other SAs,the linearity ranged in 0.01~5 μg/ml.The detection limits of SAs were 2.0~5.0 μg/kg (S/N=3).At the spiked levels of 10 and 40μg/kg,the average recoveries of SAs were 70.4%～91.4％ and 73.3%～92.5％,respectively.The relative standard deviations were less than 10％.Routine tests show that the proposed separation and determination method is rapid,sensitive and efficient and indicate that it is practical for the determination of SAs in aquatic products.
