Abstract:Myostatin is a member of the transforming growth factor-β superfamily, which functions as a negative regulator for the development and growth of skeletal muscle in animal. Based on the cloned myostatin-1 gene of Larimichthys crocea, the promoter sequence of L. crocea myostatin11 gene was cloned by genomic walking. The sequencing results indicate that it shares 90% and 75% homology identity with the corresponding reported sequences of Sparus aurata and Micropterus almoides, respectively. Bioinformatic analysis showed that myostatin-1 gene contains one consensus sequences of TATA box, a CAAT box and five putative E-boxes known as the binding sites to myogenic basic helix-loop helix transcription factors. In addition, it also possesses many transcription factor binding sites, such as MyoD, USF, MEF2, SP1 and NF-Y. These results are expected to be the basis for further studies on the regulation and expression of this gene.KEY WORDSLarimichthys croceaMyostatin-1 promoterCloningBioinformatic analysis
