Abstract:The bacterial community composition in the intestine and aquaculture pond of Apostichopus japonicus cultured at Zhuanghe, Dalian was primarily analyzed with 16S rDNA PCR-DGGE fingerprint. The results showed that 41, 31 and 41 bands were observed in the PCR-DGGE fingerprint of intestine, sediment and seawater, with 12, 9 and 10 dominant bands respectively. 20 bands different in abundance were shared by intestine, sediments and seawater, and 4 of them were dominant bands in each sample. 5, 2 and 13 special bands appeared in each sample, in which band 34, 42 from intestine and band 25, 26 from water were with higher amount than the others. Similarity between the intestine and sediment was 77.88%, while 65.9% was defined between the intestine and seawater and 556% between the sediment and seawater.
