Abstract:A multiplex polymerase chain reaction (PCR) was developed for synchronous detection of three major pathogens of aquatic animals, including Vibrio anguillarum, Aeromonas hydrophila and Edwardsiella tarda. Three important and special virulent genes were selected for the multiplex PCR, such as positive transcriptional regulator toxR gene of V. anguillarum, aerolysin gene aerA, which encodes important virulent factor of A. hydrophila, and evpA gene, which encodes the apparatus protein of secretion system T6SS of E. tarda. The reaction condition was optimized and the specificity and sensitivity was tested for this method. The detection for real samples also proved its reliability.
