Abstract:As a new laboratory fish in China, Gobiocypris rarus has the potential to develop into an ornamental fish by use of the transgenic techniques.A DNA fragment of 1584bp of β-actin gene from G.rarus genome was obtained by PCR,which included a 1507bp of a regulatory region and a 77bp of partial open reading frame (ORF).The regulatory region included a 109bp of 5′proximal promoter,an untranslated exon,and an intron of β-actin gene.The proximal promoter region contained consensus sequences of TATA box, CCAAT box and CArG box which were critical for transcription activity. Moreover, the intron also contained a typical CArG box.This promoter region fragment was inserted into green fluorescent protein gene vector (pAcGFP1-1),and the recombinant is then injected into the fertilized eggs of G. rarus by the method of micro-injection.The green fluorescence was observed under micro fluoroscope. This study proves that the cloned β-actin promoter has effective transcription activity.
