Abstract:Rab gene family plays an important role for endosome in formation, transportation, anchoring and fusion of/with phagosome/lysosome.Based on the existing Rab cDNA fragment of Pinctada martensii,the total open reading frame was cloned by using RACE method.The total cDNA consists of 2519 bp,including a 618 bp coding sequence region.Analysis of deduced amino acid sequences showed that all conserved motifs and regions can be found among these 206 amino acids.Furthermore,it was suggested that this Rab gene was a Rab7 homology because its amino acids constitution was more similar to Rab7 of human beings and other organisms, than to any other Rab subfamilies. Rab7 transcripts could be detected at expected levels respectively in liver and blood of P.martensii infected or not infected with Polydora ciliate,and in mature eggs. However, nothing was found in gill,tropoda or stomach. These results suggested that Rab7 was a tissue-specific expressing gene and may be involved in special procedures.
