Abstract:Carrageenan oligosaccharide (KOS) was prepared through enzymatic hydrolysis. After chemical modification of the produced oligosaccharide via use of formamide-chlorosulfonic acid or DMSO-methanol-pyridine, sulfated derivatives (SKO) and desulfated (DSK) derivatives of κ-carrageenan oligosaccharides were obtained respectively. Herpes simply virus (HSV-1) was used to study the relationship between the content of sulfate group and antiherpesviral activities of the oligosaccharide and its derivatives. The antiherpesviral mechanism of κ-carrageenan oligosaccharide was also studied by the ways of directly inactivating virus, inhibiting virus absorption and influencing virus replication and release. The results showed that KOS and SKO had little cytotoxic effect on Vero cells, and could not directly inactivate HSV-1 or influence its replication and release, but absorption of HSV-1 on the surface of cells was inhibited. SKO had stronger effects on HSV-1 absorption than KOS with the same concentration, while DSK had no effect on HSV-1. So it could be concluded that there was a positive relationship between the content of the sulfate group of κ-carrageenan oligosaccharide and its antiherpesviral activity, which was achieved mainly via inhibiting HSV-1 absorption on the host cells.
