Transcription of turbot Scophthalmus maximus Mx gene induced by polyinosinic polycytidylic acid given in different ways

Abstract:

Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI∶C with intraperitoneal injection, immersion and oral administration. Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI∶C’s induction. The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI∶C application. The immersion method seemed to be the most effective way to induce Mx mRNA, as Mx mRNA could last more than 120 h at a high level. This experiment proved that the homemade PolyI∶C could effectively induce Mx mRNA transcription in different applications, which provided a reference to use PolyI∶C in aquaculture practice.