Determination of three nitroimidazole residues in aquatic products by high performance liquid chromatography APCI(+)/tandem mass spectrometry with internal standard method

Abstract:

The method for the determination of three nitroimidazole residues in aquatic products was developed by high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS). Deuterated dimetridazole (DMZ-D3) was added to the sample as internal standard before extraction. The sample was extracted with ethyl acetate. The mass spectrometer was operated in the positive ion mode using multiple reaction monitoring for qualitative and quantitative analysis of MTZ,DMZ and RNZ at the same time. The preliminary treatment included predigestion，and no solid phase extraction(SPE) procedure was adopted. The advantage of the method was simple operation，less organic chemicals consumed，and shorter operation time. The limits of detection for DMZ,MTZ and RNZ were 0.5 μg/kg. The limits of quantitation for DMZ、MTZ and RNZ were 1.0 μg/kg.