Abstract:Combined with artificial breeding techniques of Sepiella maindroni, embryonic development of Sepiella maindroni was observed under microscope or anatomical lens by peeling off egg membrane of fertilized eggs. Egg of S.maindroni was anisolecithal and adhesive, and black in color. Its egg membrane was tertiary egg membrane; it showed meroblastic cleavage, and was discoblastula and directive occurrence type. The incubation period was from 14 d to 23 d at temperatures of 23.8-26.2 ℃, salinity 29.3, and the peak hatching time was 18~21 d. The hatching rate was 83.1%, accounting for 79.4% of the whole amount of hatched larvae. The speed of embryonic development was positively connected with water temperature (20-30 ℃), and the hatching rate greatly decreased when salinity was lower than 22.