Genetic variation analysis of four wild populations of Portunus trituberculatus by isozyme

Abstract:

ertical polyacrylamide gel electrophoresis was used to investigate the genetic variation of isozyme of Portunus trituberbuculatus. Four wild geographic populations distributed in the Yalujiangkou(YL), Laizhou Bay(LZ), Zhoushan(ZS) and Haizhou Bay(HZ) (48 individuals each )were surveyed. Of 22 loci encoding 11 enzymes, three were polymorphic. The results showed that: (1)the mean proportion of polymorphic loci(P099) of YL, LZ, ZS and HZ were 13.6%, 13.6%, 13.6% and 13.6%, respectively ; (2) there were no significant differences in biochemical genetic characteristics of the P. trituberbuculatusamong four wild geographic populations. The genetic divergences (Dnei) among the four wild geographic populations ranged from 0.00054 to 0.00170, which were lower than that between subspecies; (3) dendrogram constructed based on the genetic distances among the four populations showed that there were two different groups: one being composed of YL and LZ and the other of ZS and HZ.