Abstract:Genetic diversity among four selective offspring stocks obtained by mating among three basic populations of Japanese flounder Paralichthys olivaceus was assessed using ten microsatellite loci. Results showed that the allele number (A) was 3-10, average allele number was 5.7, effective allele number (Ae) was 1.357 1-4.597 9, average effective allele number was 2.983 2, average observed heterozygosity (Ho) of each locus was 0.208 3-1.000 0, average expected heterozygosity (He) of each locus was 0.208 1-0.795 6, average polymorphism information content (PIC)of each locus was 0.167 1-0.7501, and polymorphism information content of JRS, RRS, RYS and JJS followed a decreasing order. Moderate and high genetic diversity was found in JJS and the other three stocks, and X2 test revealed that there was no significant difference in average observed heterozygosity of the four selective offspring stocks. Genetic distance between JJS and RRS or RYS was comparatively higher than that between RRS and RYS. The genetic distance among the four stocks was used to construct NJ dendrogram via MEGA software. RRS and RYS were within one branch, and JJS and JRS within another branch. Genetic deviation index indicated a difference among stocks. These results indicated that these microsatellite loci selected were very sensitive and could be used in further selective and genetic breeding studies of Japanese flounder.
