Expression and binding activity of vp28 of white spot syndrome virus

Abstract:

In this study, a pair of primers was designed by primer design software 50 according to the sequence of vp28 gene of white spot syndrome virus (WSSV) in GenBank. The vp28 DNA fragment was amplified by PCR and cloned into Escherichia coli (E.coli) expression vector pBAD/gⅢA successfully. The pBAD/gⅢA VP28 was then transformed into E.coli．After L arabinose induction at 37℃，the fusion protein with 31 kDa was expressed，which was confirmed by SDS PAGE and Western blot analysis. Binding assay by fluorescence microscopy in vitro showed that VP28 was capable of binding to shrimp hemocytes. Results confirmed that the recombinant vector pBAD/gⅢA VP28 can express vp28 gene of WSSV and the VP28 had high antigenic activity.