Abstract:A self designed culture system was used for the study of intensive indoor cultivation of Calanus sinicus. Water flow and temperature of the circulating system was automatically controlled. During the course of 61 d laboratory experiment, the number of adult Calanus sinicus increased from 2.3 ind/L to19.7 ind/L, and the egg production increased from14.7 eggs/L to 85.7eggs/L, respectively. Major water quality parameters, such as DO, NH4-N and CODcr were kept at an acceptable range. The results indicated that the establishment of this culture system might lay a foundation for intensive indoor cultivation of Calanus sinicus.