Abstract:The biological characteristics and phylogenetic analysis of bacteria isolated from Epinephelus lanceolatus L. were studied, including morphological characteristics, physiological and biochemical characteristics. In addition, the 16S rRNA gene were partially sequenced and compared with sequences registered in databases; and molecular phylogenetic trees were constructed. The results showed that the two pure strains （HQ061227 1、HQ061227 2）belonged to P. damselae subsp. damselae (Love et al. 1982; Smith et al. 1991) of Photobacterium (Beijerinck〓1889 ), the sequenced 16S rRNA gene of strain HQ061227 1（accession No. EF635307) is 1 469bp in length, and exhibited high similarity (99%) with the 16S rRNA gene of P. damselae from GenBank database. Antibiotic sensitivity using 37 antimicrobial agents showed that the isolates were sensitive to 32 agents including cefazolin, resistant to 4 agents including penicillin G, and slightly sensitive to ampicillin.
