Analysis of virulence and immunogenicity of the extracellularproducts (ECP) extract from Aeromonas salmonicida masoucida

Abstract:

The extracellulose products (ECP) of Aeromonas salmonicida masoucida was extracted by using cellophane plate technique. It was proved to be lethal to swordtail fish (Xiphophorus helleri), and the LD50 does was 4.72 g protein/g. The ECP was analyzed by SDS PAGE and thirteen proteins were observed. Western blot with rat's antiserum against ECP showed that seven proteins among those thirteen proteins, whose molecular weight were 88, 70, 42, 39, 36, 22 and 15 kDa,respectively, were considered to have immunogenicity and could activate the immunoreaction of the hosts to secrete antibody.