The karyotype of Platichthys stellatus was prepared for its head kidney by PHA , colchicine injection and air drying method. The karyotype was analyzed. The results showed that the chromosome number of the species was 2n=48, including 46 telocentric chromosomes and 2 sub middle chromosomes and there was no visible evidence of the heterotypical chromosome and satellites. The karyotype formula was 2n=48,2 sm+46 t,NF=50.