Abstract:The paper aims at the toxic effect of water accomodated fractions (WAFs) of petroleum hydrocarbon pollutants such as No.0 diesel oil, maritime diesel oil and maritime heavy fuel oil on eggs, sperms and embryos of sea urchin, Hemicentrotus pulcherrimus by adopting embryonic development technology. The results indicate that the fertilization rate decreased and time delayed with the increasing concentration of WAFs. The sperms were more sensitive to the three kinds of oil WAFs than the eggs, which can be taken as indicator organism. The three kinds of oil dispersions inhibited the development from fertilized eggs to 2 , 4 , 8 , 16 cell and blastocyst stage. The effect increase along with the growth of embryos and oil concentration. Toxicity of the oils is in the order of No.0diesel oil> maritime diesel oil> maritime fuel oil. And meanwhile an analysis on the components has been carried out by way of GC-MS.
