Analysis of isozymes in different shell colors of Patinopecten yessoensis

Abstract:

The electrophoretograms of isozymes from adductor muscle of Patinopecten yessoensis were analyzed by vertical polyactylamide gel electrophoresis．Results showed that there were 17 loci by detecting 8 isozymes (MDH，ADH，ME，SOD，EST，GDH，SDH，α AMY) in white scallops and brown scallops. Among the recorded loci，7 loci (Sod 2，s Mdh 1，Est 2，Est 3，Sdh 1，α Amy 2，α Amy 5) and 6 loci (Sod 2，s Mdh 1，Est 2，Sdh 1，α Amy 2，α Amy 5) were polymorphic in white and brown scallops, and the mean proportion of polymorphic loci (P) was 4118 % and 3529 %．The mean effective number of alleles per locus (Ae) was 1.142 1 and 1104 0, the mean expected heterozygosity per locus (He) was 0091 9 and 0067 4, and the mean observed heterozygosity (Ho) was 0127 5 and 0090 7. In the mean time, the deviation indexes from Hardy Weinberg equilibrium (d) of the polymorphic loci were calculated, both of them showed the absence of heterozygosity. The genetic variability of white scallops was higher than those of brown scallops, which had a better growth potential during the further selection. We conclude that white scallops have batter potential a for further selection and breeding. At the same time, we found the dissimilarity of activity and polymorphism in Est 3 between white scallops and brown scallops, and it can be used as the genetic marker of variation.