Prevention of Vibrio infection by application of Bdellovibrio bacteriovorus in intensively cultured shrimp

Abstract:

Bdellovibrio bacteriovorus strain Bd 9913 was identified using 16S rDNA universal primers 63F and 842R. The lytic ability of Bd 9913 on shrimp pathogenic bacterial agents, Vibrio alginolysticus and V. parahaemolyticus, and a standard strain of Escherichia coli was investigated respectively. The safety of Bd109-J and Bd 9913 to postlarvae of white leg shrimp Litopenaeus vannamei,was demonstrated in two weeks after oral administration of 30 105 PFU/ml Bdellovibrios mixed 1∶1(v/w) with commercial feed Disinfection effect of Vibrioes in shrimp water was compared between chemical disinfectant treatment (0.3mg/L chloride and/or iodide) and Bdellovibrios addition (30×105 PFU/ml). Results indicated that the later showed great advantage to the former in keeping the concentration of live vibrios at a safe level twice as long (20 days). Furthermore,advantage was also shown when Bdellovibrios rather than chemical treatment was used to control shrimp vibrio infection in the outdoor intensive shrimp ponds.