Abstract:The biological characteristics of bacteria isolated from oceanic whitetip shark Carcharhinus longimanus L. were examined, including morphological characteristics, physiological and biochemical characteristics and antibiotic sensitivity. In addition, the 16S rRNA gene were partially sequenced and compared with sequences listed in databases; molecular phylogenetic trees were constructed. The results showed that the eight pure strains（HQ050226-1 to HQ050226-4 and HQ050227-1 to HQ050227-4）belonged to Vibrios (V. harveyi). The sequenced 16S rRNA gene of strain HQ050226-1 (GenBank accession No. EF635305) was 1463bp in length, the sequenced 16S rRNA gene of strain HQ050227-1 (GenBank accession No. EF635306) was 1464bp in length, and exhibited high similarity (98% and 99%) to the 16S rRNA gene of V. harveyi from GenBank database. Antibiotic sensitivity using 37 antimicrobial agents showed that isolates were resistant to 4 agents including penicillin G，but were sensitive to 33 agents including cefazolin.
