RAPD analysis of genetic diversity in first generation of introduced Paralichthys lethostigma

Abstract:

RAPD technique was used to analyze the genetic diversity of forty eight fish samples from the first generation of introduced Paralichthys lethostigma. Twenty five primers screened from 40 10bp primers produced highly repetitive and steady bands. 154 DNA fragments were identified, of which 79.22% (122 fragments) were polymorphic. The mean effective number of alleles per locus was 1.562 9±0.363 1,Nei’s gene diversity index was 0.318 4±0.184 3, and Shannon's information index was 0.465 1±0.257 4. All the results indicated that P. lethostigma had high genetic variability.