Abstract:Miichthys miiuy is a high-protein, low-fat marine species of significant economic value. However, its fillets are highly susceptible to textural deterioration, reduced water-holding capacity, and flavour degradation during processing and storage, severely limiting product quality and market value. Traditional phosphate treatments offer partial quality improvement but carry health concerns and mask flavour. To develop an eco-friendly, efficient tenderising process that synergistically enhances texture and flavour, addressing the textural deterioration and reduced water-holding capacity issues during processing and storage, thereby elevating the processing quality and market competitiveness of Miichthys miiuy fillets. Using Miichthys miiuy fillets as subjects, this study employed single-factor experiments were conducted to investigate the effects of glutamine transaminase (TG enzyme), papain, polyglutamic acid, trehalose, cellulose, β-glucan addition levels, and processing time on fillet texture and sensory characteristics. The tenderization processing technique was optimized, and gas chromatography-ion mobility spectrometry (GC-IMS) was used to analyze the changes in volatile flavor compounds under different treatments, identify key flavor compounds, and clarify their correlations. Compared to phosphate treatment (chewiness:1.13 mJ), the optimized process reduced chewiness from 11 mJ to 0.57 mJ and decreased hardness by 89.1%. The tenderizing effect of the optimized process on croaker fillets was significantly greater than that of phosphate treatment. The optimized process established in this study not only significantly outperforms conventional phosphate treatment in terms of texture improvement but also precisely modulates the profile of volatile flavor compounds, effectively masking fishy off-notes while enhancing characteristic desirable aromas. This dual functionality enables a comprehensive enhancement of Miichthys miiuy fillet quality and provides a scientific foundation for its industrial-scale processing.